Bacteriological Analysis of Water Samples from Different Points in a Tertiary Care HospitalCorrespondence Address :
Mr. Yashraj Kirankumar,
112, Surabhi, 3rd Main, 5th Stage, BEML Layout, Next to Swargarani Church, Rajarajeshwari Nagar, Bangalore-560098, Karnataka, India.
Introduction: Water is a critical component of public health, and failure to supply safe water will place a heavy burden on humanity. Drinking water is the major source of microbial pathogens in developing regions. Water contamination can be a major source of infection in a hospital.
Aim: To determine the percentage of contamination (if contamination is present) with calculation of prevalence of bacteria and to analyse the bacteriological profile of the organisms present.
Materials and Methods: Hi Media test kit was used to collect water samples from 20 different points in the hospital and was subjected to incubation for 24-48 hours. The bottles that showed colour change were further subjected to culture and sensitivity with identification of organism.
Results: There was 100% contamination of the water samples and the organisms identified include E.coli, Klebsiella, Enterobacter, Citrobacter, Proteus, NF GNB, Salmonella, Pseudomonas and Acinetobacter. Some of these organisms and isolates were found to be resistant to Cefuroxime, Ampicillin, Amoxicillin- clavulanic acid, Cefoperazone, Imipenem.
Conclusion: These results provoked the authors to reconsider the efficacy of the existing water purification systems and analyse where the point of contamination is, take corrective measures and use the apt method of purification systems to improve the quality of life of the patients.
Coliform, Communicable diseases, Culture and sensitivity, Drug resistance, Water contamination
Yashraj Kirankumar, Giridhara Upadhyaya, Vasundhara Bhatia. Bacteriological Analysis of Water Samples from Different Points in a Tertiary Care Hospital. Journal of Clinical and Diagnostic Research [serial online] 2019 November [cited: 2020 Jan 25 ]; 13:DC04-DC09. Available from
Date of Submission: Apr 24, 2019
Date of Peer Review: Jun 17, 2019
Date of Acceptance: Jul 30, 2019
Date of Publishing: Nov 01, 2019
â€¢ Financial or Other Competing Interests: No
â€¢ Was Ethics Committee Approval obtained for this study? Yes
â€¢ Was informed consent obtained from the subjects involved in the study? No
â€¢ For any images presented appropriate consent has been obtained from the subjects. No
PLAGIARISM CHECKING METHODS: [Jain H et al.]
â€¢ Plagiarism X-checker: Apr 25, 2019
â€¢ Manual Googling: Jul 18, 2019
â€¢ iThenticate Software: Oct 10, 2019 (6%)
Etymology: Author Origin
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